Early Endosome Fusion Assay
With this in vitro assay we queried the necessary components for endosomal fusion in Ohya et al. (2009).
MotionTracking is a collection of visualization, processing and image analysis tools. We started the project in 2001 to track the movement of intracellular vesicles and later expanded it to high-performance calculations of high-throughput genome-wide screening data and liver tissue 3D analysis. We build MotionTracking as a language-stack paradigm, where semantically different parts of applications are implemented based on different programming languages:
The general logic flow of data processing, algorithms, and user interface in the dynamic object-oriented language Pluk, number crunching algorithms in C++ and OpenCL, and the interface of the operating system GUI and object-oriented database access in specialized declarative languages (OWML and ODML).
This approach automates code generation and accelerates the prototyping and logic design of algorithms. Thus, MotionTracking can extract more than 100 different statistical parameters from microscopy data.