Principles of cell and tissue organization


APPL endosomes are not obligatory endocytic intermediates but act as stable cargo-sorting compartments

In this study we used a combination of imaging, quantitative microscopy analysis and modeling to investigate the role of APPL1 in early endosomes. We analyzed the ultrastructural morphology, kinetics of cargo transport, and stability of the APPL1 compartment over time and found that APPL1 resides on a tubulo-vesicular compartment that displays typical features of early endosomes (i.e. sorting cargo for recycling or degradation, long lifetimes). Our data suggest that APPL1 endosomes represent a distinct population of Rab5-positive sorting endosomes, thus providing important insights into the compartmental organization of the early endocytic pathway. Read more about this study here.

Zerial lab